![]() ConclusionĪssembled in a single user interface, SFMetrics serves as a semi-automatic analysis tool capable of measuring several geometrical properties (length, volume and angles) from DNA and protein complexes, but is also applicable to other samples with irregular shapes. ![]() We present SFMetrics as a metrology toolbox for SFM, specifically aimed at biomolecules like DNA and proteins, which features (a) semi-automatic high-throughput analysis of individual molecules (b) ease of use working within MATLAB environment or as a stand-alone application (c) compatibility with MultiMode (Bruker), NanoWizard (JPK instruments), Asylum (Asylum research), ASCII, and TIFF files, that can be adjusted with minor modifications to other formats. ![]() Most existing SFM software tools are aimed at broad general use: from material-surface analysis to visualization of biomolecules. However, high-throughput analysis of SFM data is limited by the lack of versatile software tools accessible to SFM users. ![]() Scanning force microscopy (SFM) allows direct, rapid and high-resolution visualization of single molecular complexes irregular shapes and differences in sizes are immediately revealed by the scanning tip in three-dimensional images. ![]()
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